Validation strategies are typically required to alleviate and eventually repair the undesired errors that may arise in a microarray experiment.One of the validation techniques widely accepted and used worldwide is the quantitative Reverse Transcriptase Polymerase Chain Reaction (RT-q PCR).
q PCR and, more specifically, real-time q PCR has become a routine and robust approach for measuring the expression of genes of interest, validating microarray experiments, and monitoring biomarkers.
This chapter will provide succinct introductions to microarray technologies applied to microbial research and fundamental notions regarding RT-q PCR and its use to validate microarray results.
A discussion including advantages and disadvantages of microbial microarray validation using RT-q PCR will be presented and current and future trends and research directions will be summarized towards the end of the chapter. Although carefully collected, accuracy cannot be guaranteed. Differing provisions from the publisher's actual policy or licence agreement may be applicable.
viral load), this review focuses on the use of q PCR for quantitation of RNA levels (2,3).
A number of early q PCR methods relying on end point analysis of PCR products were proposed soon after its development (4,5,6).
As can be seen in Figure 1, citations of q PCR increased dramatically (following a brief lag during adoption of the technology) with the introduction of real-time q PCR.